The analytical method was developed for the simultaneous determination of main alkaloids in poppy straw using high-performance liquid chromatography. The optimal chromatographic conditions and the sample preparation technique, including solid phase extraction, were designed and validated. The validation parameter testing included the verification of the repeatability, accuracy, linearity, sensitivity, limit of detection, limit of quantification and robustness of the analytical method. The newly developed method was applied to analyse real poppy straw samples from the harvest of 2013. The results show that this method can be used as a routine, reliable and accurate technique to determine the poppy straw quality in poppy breeding.
Keywords: - Poppy; Poppy straw; Morphine; Alkaloids; Extraction; HPLC; Validation
Undoubtedly, the wireless sensor networks (WSN) is one of the important topics in recent years, where there were many studies have focused on some topics and omission others. Because WSN dealing with limited resources, such as a small memory and limited power, improving these determinants accounted for developers and researchers. This article compared the most common cluster based routing algorithms In many respects and highlights the advantages and weaknesses of each algorithm to serve as a guide for developers to choose What fits with them requirements. Furthermore, this article provides advice and guidance for future topics and highlights neglected aspects in this topic.
Keywords— Node sensors; Clustering protocols; routing; energy efficiency;
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